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Binding Characterization (SPR/BLI)

A wet-lab reference skill for choosing between SPR and BLI, troubleshooting binding signal problems, and validating kinetic data quality.

UtilitiesAdvanced★ 170⑂ 28AI score 8/10Last updated: Jul 1, 2026

What it does

  • Provides a SPR vs BLI decision matrix across sensitivity, throughput, sample purity, kinetic resolution, maintenance and cost.
  • Gives symptom-driven troubleshooting tables for "BLI works but SPR doesn't" (hydrophobic CDRs, aggregation, charge mismatch) and the reverse case.
  • Explains mass transport limitation: how to recognize it (linear association, kon varying with ligand density) and how to mitigate it (<200 RU loading, 50–100 μL/min flow, oriented immobilization).
  • Ranks non-specific binding blockers (BSA, Tween-20, trehalose, sucrose, CM-dextran, NaCl) with working concentrations plus pH-vs-pI rules.
  • Supplies a regeneration scouting order and a kinetics QC checklist with explicit red flags (chi², kon/koff error bounds, KD consistency).

Who it's for

  • Protein engineers and antibody developers running binder screens
  • Lab scientists operating Biacore, Octet or similar instruments
  • CRO/QC reviewers who must judge whether published kinetics are trustworthy

Examples

  1. "Should I measure this antibody's KD by SPR or BLI?" → get a platform recommendation based on sample purity and throughput needs.
  2. "My sensorgram rises during the dissociation phase" → diagnose ligand leaching or reference drift and switch to capture-based immobilization.
  3. "kon changes when I load more ligand" → identified as mass transport limitation, with density/flow-rate fixes and kt-model fitting advice.

· · · Install guide · · ·

Try it now, no install

Paste this into Claude to use the skill without installing anything.

Read the instructions in this file and follow them to help me:
https://raw.githubusercontent.com/BioTender-max/awesome-bio-agent-skills/HEAD/skills/adaptyv/binding-characterization/SKILL.md

What I want: (describe your task here)

If Claude can't open the link, open it yourself and paste the contents instead.

↓ If it works for you, download the ZIP below and install it. Then it runs on its own — no pasting each time.

Install in the Claude app (no terminal)
  1. Download the ZIP with the button below.
  2. In Claude, open Settings → Capabilities and turn on 'Code execution and file creation'. (one time)
  3. Go to Customize → Skills → + → 'Upload a skill' and upload the ZIP.
↓ Download ZIP
Install in Claude Code

Let Claude do it — paste this into Claude Code

Install the skill I found on Claude Skill Mart.
Copy the skills/adaptyv/binding-characterization folder from the GitHub repo BioTender-max/awesome-bio-agent-skills into my ~/.claude/skills/binding-characterization/.
When it's done, tell me in one line what this skill can do.

Install with a command instead

git clone https://github.com/BioTender-max/awesome-bio-agent-skills.git && mkdir -p ~/.claude/skills && cp -r awesome-bio-agent-skills/skills/adaptyv/binding-characterization ~/.claude/skills/

⚠ This is a third-party skill. Check the source repository before installing.

  1. Open a terminal (Terminal on macOS/Linux, Git Bash on Windows).
  2. Create the skills folder if needed: mkdir -p ~/.claude/skills
  3. Clone the repository: git clone https://github.com/BioTender-max/awesome-bio-agent-skills.git
  4. Copy just this skill: cp -r awesome-bio-agent-skills/skills/adaptyv/binding-characterization ~/.claude/skills/
  5. Verify it landed: ls ~/.claude/skills/binding-characterization/SKILL.md
  6. Restart Claude Code and ask something like "Help me plan an SPR kinetics experiment" to trigger the skill.
View source on GitHub ↗License: NOASSERTION